Hotspot

cysA

LSC · 52333-53415 · 1083 bp

Back to Pedinomonas

Pi 0.252

MarkerSeek score 83.40

Primer available yes

Label cysA

Feature Metadata

label_name: cysA
feature_type: gene
region: LSC
length_bp: 1083
variable_sites: 256
pi: 0.252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cysA_p1	TGCTGGTCGTCGAGTTATTG	ACTCAGTTGGTAGAGTGGCT	50.0	50.0	58.0	58.0	2399 bp	0.5	59.8
cysA_p2	AAGATGCGCCATTTAGACGT	ACTCAGTTGGTAGAGTGGCT	45.0	50.0	58.0	58.0	2247 bp	0.5	59.7
cysA_p3	AGATGCGCCATTTAGACGTT	ACTCAGTTGGTAGAGTGGCT	45.0	50.0	58.0	58.0	2246 bp	0.5	59.7
cysA_p4	TGCTGGTCGTCGAGTTATTG	GCGGATGTTGCATAGTGGTA	50.0	50.0	58.0	58.1	1738 bp	0.5	59.5
cysA_p5	AGATGCGCCATTTAGACGTT	GCGGATGTTGCATAGTGGTA	45.0	50.0	58.0	58.1	1585 bp	0.5	59.4