Hotspot

chlI

LSC · 64012-65118 · 1107 bp

Back to Pedinomonas

Pi 0.164

MarkerSeek score 83.10

Primer available yes

Label chlI

Feature Metadata

label_name: chlI
feature_type: gene
region: LSC
length_bp: 1107
variable_sites: 174
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI_p1	TTCCAAAGGAAAGGTGTCCG	GATCAGGTGGTGCTAGTTGG	50.0	55.0	58.0	58.0	2169 bp	0.5	59.6
chlI_p2	TAGATGCCATTGCGATTGCT	GATCAGGTGGTGCTAGTTGG	45.0	55.0	58.0	58.0	1954 bp	0.5	59.5
chlI_p3	GAAAGGTGTCCGAGTGGTTT	GATCAGGTGGTGCTAGTTGG	50.0	55.0	58.0	58.0	2161 bp	0.5	59.5
chlI_p4	AAACAGCAAGCCGTACTTCA	GATCAGGTGGTGCTAGTTGG	45.0	55.0	58.0	58.0	2407 bp	0.5	59.5
chlI_p5	GTTCGAATCCCTCCCTTTCC	GATCAGGTGGTGCTAGTTGG	55.0	55.0	58.0	58.0	2098 bp	0.5	59.4