Hotspot

atpA

LSC · 149-1669 · 1521 bp

Back to Pedinomonas

Pi 0.134

MarkerSeek score 82.60

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 181
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ACCTGACCAACTAAGTTGCT	GCTAACCCATTCGCAGGTTA	45.0	50.0	56.7	58.0	2337 bp	0.5	49.1
atpA_p2	ACCTGACCAACTAAGTTGCT	AGCCAACAAGTAATTCGCCA	45.0	45.0	56.7	58.1	1901 bp	0.5	48.6
atpA_p3	ACCTGACCAACTAAGTTGCT	GTCATGGTCACGGATTTGGT	45.0	50.0	56.7	58.2	2176 bp	0.5	48.0
atpA_p4	ACCTGACCAACTAAGTTGCT	CAATTGGTCATGGTCACGGA	45.0	50.0	56.7	58.2	2182 bp	0.5	48.0
atpA_p5	ACCTGACCAACTAAGTTGCT	TGGTCATGGTCACGGATTTG	45.0	50.0	56.7	58.2	2178 bp	0.5	48.0