Hotspot

orf105-psbA

IRb · 70302-71124 · 823 bp

Back to Pedinomonas

Pi 0.416

MarkerSeek score 81.60

Primer available yes

Label orf105-psbA

Feature Metadata

label_name: orf105-psbA
feature_type: igs
region: IRb
length_bp: 823
variable_sites: 332
pi: 0.416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf105-psbA_p1	AAAAAGGCCCTGTACTGTGG	CTTACATGGGTCGTGAGTGG	50.0	55.0	58.0	58.0	2203 bp	0.5	59.9
orf105-psbA_p2	AAAAAGGCCCTGTACTGTGG	ATGCACCCATTCCACATGTT	50.0	45.0	58.0	58.1	1997 bp	0.5	59.5
orf105-psbA_p3	AAAAAGGCCCTGTACTGTGG	GGTGTAGCAGGTGTATTCGG	50.0	55.0	58.0	58.1	1976 bp	0.5	59.5
orf105-psbA_p4	AAAAAGGCCCTGTACTGTGG	GTAGCAGGTGTATTCGGTGG	50.0	55.0	58.0	58.1	1973 bp	0.5	59.5
orf105-psbA_p5	AAAAAGGCCCTGTACTGTGG	GGAAGTAATGCACGAGCGTA	50.0	50.0	58.0	58.1	1593 bp	0.5	59.3