Hotspot

psbC-orf105

LSC · 69357-69983 · 627 bp

Back to Pedinomonas

Pi 0.402

MarkerSeek score 80.40

Primer available yes

Label psbC-orf105

Feature Metadata

label_name: psbC-orf105
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 248
pi: 0.402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-orf105_p1	CGAGTTCTACGGTCCAACTG	AGGTGCCTAGAGATTCACGA	55.0	50.0	58.0	57.9	1608 bp	0.5	58.7
psbC-orf105_p2	TGACCAACGTTTAGGTGCAA	AGGTGCCTAGAGATTCACGA	45.0	50.0	58.0	57.9	1545 bp	0.5	58.5
psbC-orf105_p3	CAGCGGCTGGTTTTGAAAAA	AGGTGCCTAGAGATTCACGA	45.0	50.0	58.1	57.9	1153 bp	0.5	58.3
psbC-orf105_p4	ATGGCGTTCATTGCTTGTTG	AGGTGCCTAGAGATTCACGA	45.0	50.0	57.9	57.9	1661 bp	0.5	58.3
psbC-orf105_p5	GTTCTACGGTCCAACTGGTC	AGGTGCCTAGAGATTCACGA	55.0	50.0	57.9	57.9	1605 bp	0.5	58.2