Hotspot

psaJ-trnP(ugg)

LSC · 33783-34253 · 471 bp

Back to Pedinomonas

Pi 0.421

MarkerSeek score 80.70

Primer available yes

Label psaJ-trnP(ugg)

Feature Metadata

label_name: psaJ-trnP(ugg)
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 189
pi: 0.421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP(ugg)_p1	AGCTTCTTTCTAGCCGAACG	TACCAAACTGCGCTACATCC	50.0	50.0	58.0	58.0	819 bp	0.5	59.8
psaJ-trnP(ugg)_p2	AGCTTCTTTCTAGCCGAACG	AACCAAGTGCAAAAACTGGC	50.0	45.0	58.0	58.0	1280 bp	0.5	59.8
psaJ-trnP(ugg)_p3	GCTTCTTTCTAGCCGAACGA	TACCAAACTGCGCTACATCC	50.0	50.0	58.0	58.0	818 bp	0.5	59.8
psaJ-trnP(ugg)_p4	GCTTCTTTCTAGCCGAACGA	AACCAAGTGCAAAAACTGGC	50.0	45.0	58.0	58.0	1279 bp	0.5	59.8
psaJ-trnP(ugg)_p5	AGCTTCTTTCTAGCCGAACG	AATGCAGCAGCTCGTGAATA	50.0	45.0	58.0	58.0	1466 bp	0.5	59.8