Hotspot

rps16

LSC · 5331-6445 · 1115 bp

Back to Pedicularis

Pi 0.0522

MarkerSeek score 81.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1115
variable_sites: 219
pi: 0.0522
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTTGTCGTTCTAGGCCATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1894-1989 bp	0.692308	79.0
rps16_p2	TGTCGTTCTAGGCCATCGTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1892-1987 bp	0.615385	74.0
rps16_p3	TTTGTCGTTCTAGGCCATCG	ATGTAACGATTGAGGCAGGG	50.0	50.0	58.0	57.7	1619-1658 bp	0.538462	70.8
rps16_p4	AAACACAAACCCGGTTCACT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	2362-2391 bp	0.153846	50.8
rps16_p5	AACACAAACCCGGTTCACTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	2361-2390 bp	0.153846	50.8