Hotspot

psaC-rps15

SSC · 114082-118570 · 4489 bp

Back to Pedicularis

Pi 0.0863

MarkerSeek score 77.60

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 4489
variable_sites: 1520
pi: 0.0863
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	AGGGCCAAGTATCCATACGA	CAGGCTCTGGAAGGAATTCC	50.0	55.0	57.9	58.0	345-466 bp	0.461538	69.3
psaC-rps15_p2	CGTTGTTGTATCCCTGCAGA	GAGCTTCCGGAGTACAATGG	50.0	55.0	57.9	58.1	227-692 bp	0.230769	60.0
psaC-rps15_p3	CGTTGTTGTATCCCTGCAGA	CAGGCTCTGGAAGGAATTCC	50.0	55.0	57.9	58.0	530-552 bp	0.307692	59.9
psaC-rps15_p4	AGTAGTTTTGTACCGTCCGC	CAGGCTCTGGAAGGAATTCC	50.0	55.0	57.9	58.0	560-606 bp	0.230769	58.5
psaC-rps15_p5	AGTAGTTTTGTACCGTCCGC	GAGCTTCCGGAGTACAATGG	50.0	55.0	57.9	58.1	715-751 bp	0.153846	53.1