Hotspot

trnL-UAG-psaC

SSC · 111046-113835 · 2790 bp

Back to Pedicularis

Pi 0.0756

MarkerSeek score 82.00

Primer available yes

Label trnL-UAG-psaC

Feature Metadata

label_name: trnL-UAG-psaC
feature_type: igs
region: SSC
length_bp: 2790
variable_sites: 861
pi: 0.0756
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-psaC_p1	TAGACACGCTGCTCTTAGGA	AACAAATCGCTTCTGCTCCA	50.0	45.0	57.9	58.1	2420-2993 bp	0.461538	68.6
trnL-UAG-psaC_p2	TAGACACGCTGCTCTTAGGA	ACAAATCGCTTCTGCTCCAA	50.0	45.0	57.9	58.1	2419-2992 bp	0.461538	68.6
trnL-UAG-psaC_p3	TGAATTCAAGACAAGCCGCT	ATTTCTTGAGCGTTCGGGTT	45.0	45.0	58.1	58.1	2381-2949 bp	0.384615	65.7
trnL-UAG-psaC_p4	TAGACACGCTGCTCTTAGGA	ATTTCTTGAGCGTTCGGGTT	50.0	45.0	57.9	58.1	2348-2916 bp	0.384615	65.5
trnL-UAG-psaC_p5	ACAAGCCGCTATGGTGAAAT	ATTTCTTGAGCGTTCGGGTT	45.0	45.0	57.9	58.1	2371-2939 bp	0.384615	65.5