Hotspot

trnN-GUU-trnL-UAG

SSC · 107722-110965 · 3244 bp

Back to Pedicularis

Pi 0.129

MarkerSeek score 75.50

Primer available yes

Label trnN-GUU-trnL-UAG

Feature Metadata

label_name: trnN-GUU-trnL-UAG
feature_type: igs
region: SSC
length_bp: 3244
variable_sites: 1350
pi: 0.129
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnL-UAG_p1	AGGCGATTAATCCTGCTGTG	GTCGTTTGTGGGCCTTTCTA	50.0	50.0	58.0	58.1	1002 bp	0.076923	41.8
trnN-GUU-trnL-UAG_p2	AGGCGATTAATCCTGCTGTG	TCGTTTGTGGGCCTTTCTAG	50.0	50.0	58.0	57.8	1001 bp	0.076923	41.3
trnN-GUU-trnL-UAG_p3	AGGCGATTAATCCTGCTGTG	TTTCATTCCACTCCCGGTTC	50.0	50.0	58.0	57.8	1077 bp	0.076923	41.2
trnN-GUU-trnL-UAG_p4	AGGCGATTAATCCTGCTGTG	CTTTCATTCCACTCCCGGTT	50.0	50.0	58.0	57.8	1078 bp	0.076923	41.1
trnN-GUU-trnL-UAG_p5	AGGCGATTAATCCTGCTGTG	GGGGTGGGGCTTCTTATTTT	50.0	50.0	58.0	57.8	1045 bp	0.076923	40.8