Hotspot

psbE-petL

LSC · 64904-65731 · 828 bp

Back to Pedicularis

Pi 0.0517

MarkerSeek score 83.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 148
pi: 0.0517
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCAGTAGAAAACCAAAATAACT	50.0	31.8	57.8	52.3	892-976 bp	1.0	52.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CCAGTAGAAAACCAAAATAACTAGT	50.0	32.0	57.8	55.3	892-976 bp	1.0	52.0
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	CCAGTAGAAAACCAAAATAACT	55.0	31.8	58.2	52.3	887-971 bp	1.0	52.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CCAGTAGAAAACCAAAATAACTAGT	55.0	32.0	58.2	55.3	887-971 bp	1.0	52.0
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	CCAGTAGAAAACCAAAATAACT	50.0	31.8	58.4	52.3	885-969 bp	1.0	52.0