Hotspot

ycf4-cemA

LSC · 60655-61206 · 552 bp

Back to Pedicularis

Pi 0.0518

MarkerSeek score 81.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 104
pi: 0.0518
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GACCCGCACTGATGAGAATT	GGGGCGAATGAAATCCGATA	50.0	50.0	58.0	57.8	1148-1348 bp	1.0	89.5
ycf4-cemA_p2	GCAAGGGATCGTGATGTCTT	GGGGCGAATGAAATCCGATA	50.0	50.0	58.0	57.8	1427-1627 bp	1.0	88.9
ycf4-cemA_p3	CAAGTATGGGTTGGCGATCA	GGGGCGAATGAAATCCGATA	50.0	50.0	58.0	57.8	1614-1814 bp	0.923077	85.5
ycf4-cemA_p4	GACCCGCACTGATGAGAATT	TGACCAGCATTCGACCAATT	50.0	45.0	58.0	57.8	774-974 bp	0.846154	84.0
ycf4-cemA_p5	GACCCGCACTGATGAGAATT	TTGACCAGCATTCGACCAAT	50.0	45.0	58.0	57.8	775-975 bp	0.846154	84.0