Hotspot

ycf3-trnS-GGA

LSC · 44150-45000 · 851 bp

Back to Pedicularis

Pi 0.0545

MarkerSeek score 83.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 152
pi: 0.0545
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGCGATCTAGACA	AACCGCTCAGCCATCTCT	45.5	55.6	57.8	58.3	840-894 bp	0.923077	53.3
ycf3-trnS-GGA_p2	CCATTTATCCGCGATCTAGACA	AACCGCTCAGCCATCTCTC	45.5	57.9	57.8	59.5	840-894 bp	0.923077	51.7
ycf3-trnS-GGA_p3	TCCATTTATCCGCGATCTAGAC	AACCGCTCAGCCATCTCT	45.5	55.6	57.6	58.3	841-895 bp	0.923077	51.2
ycf3-trnS-GGA_p4	TCCATTTATCCGCGATCTAGAC	AACCGCTCAGCCATCTCTC	45.5	57.9	57.6	59.5	841-895 bp	0.923077	49.6
ycf3-trnS-GGA_p5	CCATTTATCCGCGATCTAGACA	ACCGCTCAGCCATCTCTC	45.5	61.1	57.8	58.8	839-893 bp	0.923077	49.2