Hotspot

psbC-trnS-UGA

LSC · 35000-35244 · 245 bp

Back to Pedicularis

Pi 0.0895

MarkerSeek score 80.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 245
variable_sites: 58
pi: 0.0895
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	340-383 bp	1.0	90.5
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	320-363 bp	1.0	89.7
psbC-trnS-UGA_p3	GCAGCCGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	379-422 bp	1.0	89.5
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	271-314 bp	1.0	89.0
psbC-trnS-UGA_p5	GCAGCCGGATTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.3	57.9	359-402 bp	1.0	88.7