Hotspot

petN-psbM

LSC · 29009-29919 · 911 bp

Back to Pedicularis

Pi 0.0544

MarkerSeek score 87.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 183
pi: 0.0544
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCTTTTCTACT	50.0	42.9	58.2	56.4	1000-1052 bp	1.0	69.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCT	50.0	45.0	58.2	55.4	1007-1059 bp	1.0	69.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCTT	50.0	42.9	58.2	56.2	1007-1059 bp	1.0	68.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACGGCT	50.0	42.9	58.2	56.2	1008-1060 bp	1.0	68.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCTTTTCTACTT	50.0	40.9	58.2	57.1	1010-1052 bp	0.923077	64.6