Hotspot

trnC-GCA-petN

LSC · 28088-28918 · 831 bp

Back to Pedicularis

Pi 0.0479

MarkerSeek score 88.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 152
pi: 0.0479
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.1	58.1	935-1091 bp	0.923077	87.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	870-1026 bp	0.923077	87.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.4	58.1	953-1109 bp	0.846154	81.9
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	888-1044 bp	0.846154	81.8
trnC-GCA-petN_p5	CCAGTTCAAATCCGGGTGTC	TTCTAGAGCCCACTTCTCCC	55.0	55.0	58.8	58.1	930-1086 bp	0.923077	81.2