Hotspot

trnS-GCU-trnS-CGA

LSC · 8438-9159 · 722 bp

Back to Pedicularis

Pi 0.0522
MarkerSeek score 81.40
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
722
variable_sites
124
pi
0.0522
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.90
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1412-1486 bp 0.923077 88.6
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1411-1485 bp 0.923077 88.6
trnS-GCU-trnS-CGA_p3 CAATCCGACGCTTTAGTCCA CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1409-1483 bp 0.923077 88.4
trnS-GCU-trnS-CGA_p4 AATCCGACGCTTTAGTCCAC CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1408-1482 bp 0.923077 88.4
trnS-GCU-trnS-CGA_p5 ATCTAATGATCCGGGGCGTA CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.1 58.0 1616-1697 bp 0.923077 88.1