primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	rps16	1	TTTGTCGTTCTAGGCCATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	5.0	16.9	0.080684	5331	6445	1894-1989	0.692308	79.0
rps16_p2	rps16	2	TGTCGTTCTAGGCCATCGTA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	5.0	16.9	0.322171	5331	6445	1892-1987	0.615385	74.0
rps16_p3	rps16	3	TTTGTCGTTCTAGGCCATCG	ATGTAACGATTGAGGCAGGG	20	20	50.0	50.0	58.0	57.7	5.0	0.0	0.336646	5331	6445	1619-1658	0.538462	70.8
rps16_p4	rps16	4	AAACACAAACCCGGTTCACT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.2	58.1	2.5	16.9	0.234049	5331	6445	2362-2391	0.153846	50.8
rps16_p5	rps16	5	AACACAAACCCGGTTCACTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.2	58.1	2.5	16.9	0.234049	5331	6445	2361-2390	0.153846	50.8
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034421	8438	9159	1412-1486	0.923077	88.6
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	8438	9159	1411-1485	0.923077	88.6
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	CAATCCGACGCTTTAGTCCA	CGTTAGCTTGGAAGGCTAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.063258	8438	9159	1409-1483	0.923077	88.4
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AATCCGACGCTTTAGTCCAC	CGTTAGCTTGGAAGGCTAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064646	8438	9159	1408-1482	0.923077	88.4
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	ATCTAATGATCCGGGGCGTA	CATCGTTAGCTTGGAAGGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096817	8438	9159	1616-1697	0.923077	88.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTCTAGAGCCCACTTCTCCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.214765	28088	28918	935-1091	0.923077	87.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28088	28918	870-1026	0.923077	87.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	TTCTAGAGCCCACTTCTCCC	20	20	50.0	55.0	58.4	58.1	14.7	0.0	0.488128	28088	28918	953-1109	0.846154	81.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28088	28918	888-1044	0.846154	81.8
trnC-GCA-petN_p5	trnC-GCA-petN	5	CCAGTTCAAATCCGGGTGTC	TTCTAGAGCCCACTTCTCCC	20	20	55.0	55.0	58.8	58.1	0.0	0.0	0.967367	28088	28918	930-1086	0.923077	81.2
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCTTTTCTACT	20	21	50.0	42.9	58.2	56.4	0.0	0.0	2.762471	29009	29919	1000-1052	1.000000	69.9
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCT	20	20	50.0	45.0	58.2	55.4	0.0	0.0	2.781070	29009	29919	1007-1059	1.000000	69.8
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCTT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	29009	29919	1007-1059	1.000000	68.2
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACGGCT	20	21	50.0	42.9	58.2	56.2	0.0	0.0	2.973013	29009	29919	1008-1060	1.000000	68.2
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACGGCTTTTCTACTT	20	22	50.0	40.9	58.2	57.1	0.0	0.0	3.037844	29009	29919	1010-1052	0.923077	64.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	59.1	57.9	0.0	0.0	1.193914	30935	31572	620-710	1.000000	82.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	19	20	57.9	50.0	58.4	57.9	0.0	0.0	1.499420	30935	31572	619-709	1.000000	80.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CCTAAACCACTAGACGATGGG	TACCGCTGAGTTAAAAGGGC	21	20	52.4	50.0	57.5	57.9	0.0	0.0	1.557909	30935	31572	623-713	1.000000	79.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CTAAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	21	20	52.4	50.0	57.5	57.9	0.0	0.0	1.557909	30935	31572	622-712	1.000000	79.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCCTCCTTGAAAGAGAGATGT	TACCGCTGAGTTAAAAGGGC	21	20	47.6	50.0	57.4	57.9	9.0	0.0	1.729187	30935	31572	644-734	1.000000	78.2
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.181932	35000	35244	340-383	1.000000	90.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.284854	35000	35244	320-363	1.000000	89.7
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCAGCCGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	58.3	58.0	5.0	0.0	0.307758	35000	35244	379-422	1.000000	89.5
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	20	20	55.0	55.0	58.2	58.2	0.0	17.2	0.377505	35000	35244	271-314	1.000000	89.0
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCAGCCGGATTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	20	20	50.0	50.0	58.3	57.9	5.0	0.0	0.410680	35000	35244	359-402	1.000000	88.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCCGCGATCTAGACA	AACCGCTCAGCCATCTCT	22	18	45.5	55.6	57.8	58.3	8.2	0.0	4.455290	44150	45000	840-894	0.923077	53.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCCGCGATCTAGACA	AACCGCTCAGCCATCTCTC	22	19	45.5	57.9	57.8	59.5	8.2	0.0	4.654692	44150	45000	840-894	0.923077	51.7
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCCATTTATCCGCGATCTAGAC	AACCGCTCAGCCATCTCT	22	18	45.5	55.6	57.6	58.3	8.2	0.0	4.717121	44150	45000	841-895	0.923077	51.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCCATTTATCCGCGATCTAGAC	AACCGCTCAGCCATCTCTC	22	19	45.5	57.9	57.6	59.5	8.2	0.0	4.916523	44150	45000	841-895	0.923077	49.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCATTTATCCGCGATCTAGACA	ACCGCTCAGCCATCTCTC	22	18	45.5	61.1	57.8	58.8	8.2	0.0	4.959166	44150	45000	839-893	0.923077	49.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46401	47099	735-785	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46401	47099	743-793	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46401	47099	767-817	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	46401	47099	775-825	1.000000	89.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46401	47099	749-799	1.000000	89.0
ycf4-cemA_p1	ycf4-cemA	1	GACCCGCACTGATGAGAATT	GGGGCGAATGAAATCCGATA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.208750	60655	61206	1148-1348	1.000000	89.5
ycf4-cemA_p2	ycf4-cemA	2	GCAAGGGATCGTGATGTCTT	GGGGCGAATGAAATCCGATA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.208750	60655	61206	1427-1627	1.000000	88.9
ycf4-cemA_p3	ycf4-cemA	3	CAAGTATGGGTTGGCGATCA	GGGGCGAATGAAATCCGATA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.215870	60655	61206	1614-1814	0.923077	85.5
ycf4-cemA_p4	ycf4-cemA	4	GACCCGCACTGATGAGAATT	TGACCAGCATTCGACCAATT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.228665	60655	61206	774-974	0.846154	84.0
ycf4-cemA_p5	ycf4-cemA	5	GACCCGCACTGATGAGAATT	TTGACCAGCATTCGACCAAT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.228665	60655	61206	775-975	0.846154	84.0
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	CCAGTAGAAAACCAAAATAACT	20	22	50.0	31.8	57.8	52.3	24.9	0.0	7.854968	64904	65731	892-976	1.000000	52.0
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	CCAGTAGAAAACCAAAATAACTAGT	20	25	50.0	32.0	57.8	55.3	24.9	0.0	7.892104	64904	65731	892-976	1.000000	52.0
psbE-petL_p3	psbE-petL	3	GTTCTCCTGTGCTTCCAGAC	CCAGTAGAAAACCAAAATAACT	20	22	55.0	31.8	58.2	52.3	0.9	0.0	7.896556	64904	65731	887-971	1.000000	52.0
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	CCAGTAGAAAACCAAAATAACTAGT	20	25	55.0	32.0	58.2	55.3	0.9	0.0	7.933692	64904	65731	887-971	1.000000	52.0
psbE-petL_p5	psbE-petL	5	TCTCCTGTGCTTCCAGACAT	CCAGTAGAAAACCAAAATAACT	20	22	50.0	31.8	58.4	52.3	0.9	0.0	8.049304	64904	65731	885-969	1.000000	52.0
trnN-GUU-trnL-UAG_p1	trnN-GUU-trnL-UAG	1	AGGCGATTAATCCTGCTGTG	GTCGTTTGTGGGCCTTTCTA	20	20	50.0	50.0	58.0	58.1	9.9	4.5	0.154072	108933	109052	1002-1002	0.076923	41.8
trnN-GUU-trnL-UAG_p2	trnN-GUU-trnL-UAG	2	AGGCGATTAATCCTGCTGTG	TCGTTTGTGGGCCTTTCTAG	20	20	50.0	50.0	58.0	57.8	9.9	4.5	0.219870	108933	109052	1001-1001	0.076923	41.3
trnN-GUU-trnL-UAG_p3	trnN-GUU-trnL-UAG	3	AGGCGATTAATCCTGCTGTG	TTTCATTCCACTCCCGGTTC	20	20	50.0	50.0	58.0	57.8	9.9	0.0	0.235160	108933	109052	1077-1077	0.076923	41.2
trnN-GUU-trnL-UAG_p4	trnN-GUU-trnL-UAG	4	AGGCGATTAATCCTGCTGTG	CTTTCATTCCACTCCCGGTT	20	20	50.0	50.0	58.0	57.8	9.9	0.0	0.241331	108933	109052	1078-1078	0.076923	41.1
trnN-GUU-trnL-UAG_p5	trnN-GUU-trnL-UAG	5	AGGCGATTAATCCTGCTGTG	GGGGTGGGGCTTCTTATTTT	20	20	50.0	50.0	58.0	57.8	9.9	0.0	0.288658	108933	109052	1045-1045	0.076923	40.8
trnL-UAG-psaC_p1	trnL-UAG-psaC	1	TAGACACGCTGCTCTTAGGA	AACAAATCGCTTCTGCTCCA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.228057	111046	113835	2420-2993	0.461538	68.6
trnL-UAG-psaC_p2	trnL-UAG-psaC	2	TAGACACGCTGCTCTTAGGA	ACAAATCGCTTCTGCTCCAA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.228057	111046	113835	2419-2992	0.461538	68.6
trnL-UAG-psaC_p3	trnL-UAG-psaC	3	TGAATTCAAGACAAGCCGCT	ATTTCTTGAGCGTTCGGGTT	20	20	45.0	45.0	58.1	58.1	5.4	0.0	0.211571	111046	113835	2381-2949	0.384615	65.7
trnL-UAG-psaC_p4	trnL-UAG-psaC	4	TAGACACGCTGCTCTTAGGA	ATTTCTTGAGCGTTCGGGTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.232068	111046	113835	2348-2916	0.384615	65.5
trnL-UAG-psaC_p5	trnL-UAG-psaC	5	ACAAGCCGCTATGGTGAAAT	ATTTCTTGAGCGTTCGGGTT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.236561	111046	113835	2371-2939	0.384615	65.5
psaC-rps15_p1	psaC-rps15	1	AGGGCCAAGTATCCATACGA	CAGGCTCTGGAAGGAATTCC	20	20	50.0	55.0	57.9	58.0	6.2	17.3	0.146998	117341	117460	345-466	0.461538	69.3
psaC-rps15_p2	psaC-rps15	2	CGTTGTTGTATCCCTGCAGA	GAGCTTCCGGAGTACAATGG	20	20	50.0	55.0	57.9	58.1	17.4	3.3	0.151981	117341	117460	227-692	0.230769	60.0
psaC-rps15_p3	psaC-rps15	3	CGTTGTTGTATCCCTGCAGA	CAGGCTCTGGAAGGAATTCC	20	20	50.0	55.0	57.9	58.0	17.4	17.3	0.143195	117341	117460	530-552	0.307692	59.9
psaC-rps15_p4	psaC-rps15	4	AGTAGTTTTGTACCGTCCGC	CAGGCTCTGGAAGGAATTCC	20	20	50.0	55.0	57.9	58.0	0.0	17.3	0.120150	117341	117460	560-606	0.230769	58.5
psaC-rps15_p5	psaC-rps15	5	AGTAGTTTTGTACCGTCCGC	GAGCTTCCGGAGTACAATGG	20	20	50.0	55.0	57.9	58.1	0.0	3.3	0.128937	117341	117460	715-751	0.153846	53.1