Hotspot

DR496_pgp022-DR496_pgp021

IRa · 218282-218563 · 282 bp

Back to Pediastrum

Pi 0.425

MarkerSeek score 75.60

Primer available yes

Label DR496_pgp022-DR496_pgp021

Feature Metadata

label_name: DR496_pgp022-DR496_pgp021
feature_type: igs
region: IRa
length_bp: 282
variable_sites: 116
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp022-DR496_pgp021_p1	GTCGGCGAGTTTGGAGTTAT	TTGTTTTGATGCGCATCGTC	50.0	45.0	58.0	58.0	1118 bp	0.5	59.7
DR496_pgp022-DR496_pgp021_p2	GTCGGCGAGTTTGGAGTTAT	GCGAGTTTGGAATCGGAGAT	50.0	50.0	58.0	58.1	1515 bp	0.5	59.5
DR496_pgp022-DR496_pgp021_p3	GTCGGCGAGTTTGGAGTTAT	GCAGCAGCAACAATTGGATT	50.0	45.0	58.0	57.9	1225 bp	0.5	59.2
DR496_pgp022-DR496_pgp021_p4	CGAAGAAGCGAATCGAAAGC	TTGTTTTGATGCGCATCGTC	50.0	45.0	57.9	58.0	862 bp	0.5	59.2
DR496_pgp022-DR496_pgp021_p5	GAAGAAGCGAATCGAAAGCG	TTGTTTTGATGCGCATCGTC	50.0	45.0	57.9	58.0	861 bp	0.5	59.2