Hotspot

DR496_pgp024-DR496_pgp023

IRa · 215880-216284 · 405 bp

Back to Pediastrum

Pi 0.451

MarkerSeek score 79.10

Primer available yes

Label DR496_pgp024-DR496_pgp023

Feature Metadata

label_name: DR496_pgp024-DR496_pgp023
feature_type: igs
region: IRa
length_bp: 405
variable_sites: 170
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp024-DR496_pgp023_p1	GAAGAAGCACCGAAGCGATA	CCGAAGAAGAGCGAAAGGAA	50.0	50.0	58.1	57.9	955 bp	0.5	58.7
DR496_pgp024-DR496_pgp023_p2	GAAGAAGCACCGAAGCGATA	CCGCAGGCAAATTGATGAAG	50.0	50.0	58.1	58.1	704 bp	0.5	58.7
DR496_pgp024-DR496_pgp023_p3	TTTGTTTTGATGCGTGTCGG	CCGAAGAAGAGCGAAAGGAA	45.0	50.0	57.9	57.9	1158 bp	0.5	58.5
DR496_pgp024-DR496_pgp023_p4	TTTGTTTTGATGCGTGTCGG	CCGCAGGCAAATTGATGAAG	45.0	50.0	57.9	58.1	907 bp	0.5	58.4
DR496_pgp024-DR496_pgp023_p5	AAGAAGCGAAGGAGCGAAAA	CCGAAGAAGAGCGAAAGGAA	45.0	50.0	58.1	57.9	978 bp	0.5	58.4