Hotspot

rrf-rrl

IRa · 203280-203512 · 233 bp

Back to Pediastrum

Pi 0.325

MarkerSeek score 89.30

Primer available yes

Label rrf-rrl

Feature Metadata

label_name: rrf-rrl
feature_type: igs
region: IRa
length_bp: 233
variable_sites: 68
pi: 0.325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrf-rrl_p1	GTTTTCCTGCCAGACTTCCA	TTTCCTCAAGATGAGCGCTC	50.0	50.0	58.0	58.0	458-465 bp	1.0	91.7
rrf-rrl_p2	TTTCCTGCCAGACTTCCAAC	TTTCCTCAAGATGAGCGCTC	50.0	50.0	58.0	58.0	456-463 bp	1.0	91.7
rrf-rrl_p3	GTTTTCCTGCCAGACTTCCA	CTTTCCTCAAGATGAGCGCT	50.0	50.0	58.0	58.0	459-466 bp	1.0	91.7
rrf-rrl_p4	TTTCCTGCCAGACTTCCAAC	CTTTCCTCAAGATGAGCGCT	50.0	50.0	58.0	58.0	457-464 bp	1.0	91.7
rrf-rrl_p5	CTGCCAGACTTCCAACAAGT	TTTCCTCAAGATGAGCGCTC	50.0	50.0	58.0	58.0	452-459 bp	1.0	91.7