Hotspot

rps8-rpl5

SSC · 190126-191015 · 890 bp

Back to Pediastrum

Pi 0.328

MarkerSeek score 83.70

Primer available yes

Label rps8-rpl5

Feature Metadata

label_name: rps8-rpl5
feature_type: igs
region: SSC
length_bp: 890
variable_sites: 228
pi: 0.328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl5_p1	TGAACCATGCAAGCATTACG	AAAATCAAGAAGAAGGTCTTTC	45.0	31.8	57.1	52.6	950-1008 bp	1.0	52.0
rps8-rpl5_p2	TGAACCATGCAAGCATTACG	AAAAATCAAGAAGAAGGTCTTTC	45.0	30.4	57.1	53.4	951-1009 bp	1.0	52.0
rps8-rpl5_p3	CCTTTTTGAACCATGCAAGCA	AAAATCAAGAAGAAGGTCTTTC	42.9	31.8	58.2	52.6	956-1014 bp	1.0	52.0
rps8-rpl5_p4	GAACCATGCAAGCATTACGA	AAAATCAAGAAGAAGGTCTTTC	45.0	31.8	56.8	52.6	949-1007 bp	1.0	52.0
rps8-rpl5_p5	TTGAACCATGCAAGCATTACG	AAAATCAAGAAGAAGGTCTTTC	42.9	31.8	57.8	52.6	951-1009 bp	1.0	52.0