Hotspot

tufA-trnV(uac)

SSC · 151702-152405 · 704 bp

Back to Pediastrum

Pi 0.274

MarkerSeek score 84.50

Primer available yes

Label tufA-trnV(uac)

Feature Metadata

label_name: tufA-trnV(uac)
feature_type: igs
region: SSC
length_bp: 704
variable_sites: 183
pi: 0.274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tufA-trnV(uac)_p1	AGCTACAGGACGTGTTGAAC	GGACTCGAACCTACGAATGG	50.0	55.0	57.8	57.8	1894-2909 bp	1.0	89.1
tufA-trnV(uac)_p2	GGTGCAGGGGTTGTAACTAG	GGACTCGAACCTACGAATGG	55.0	55.0	57.9	57.8	1380 bp	0.5	57.5
tufA-trnV(uac)_p3	AGGAGGACGTACAGTAGGTG	GGACTCGAACCTACGAATGG	55.0	55.0	57.9	57.8	1396 bp	0.5	57.4
tufA-trnV(uac)_p4	GGTGCAGGGGTTGTAACTAG	AATGAAAGAATCTGGGGCGG	55.0	50.0	57.9	58.2	1402 bp	0.5	57.3
tufA-trnV(uac)_p5	AGGAGGACGTACAGTAGGTG	AATGAAAGAATCTGGGGCGG	55.0	50.0	57.9	58.2	1418 bp	0.5	57.1