Hotspot

psbM-trnR(acg)

SSC · 145433-146517 · 1085 bp

Back to Pediastrum

Pi 0.295
MarkerSeek score 82.70
Primer available yes
Label psbM-trnR(acg)

Feature Metadata

label_name
psbM-trnR(acg)
feature_type
igs
region
SSC
length_bp
1085
variable_sites
245
pi
0.295
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnR(acg)_p1 AACAGCAGCAAATGAATCTG CCACATGCTCTAGTCCACTG 40.0 55.0 54.9 57.7 943-1142 bp 1.0 64.4
psbM-trnR(acg)_p2 AAACAGCAGCAAATGAATCTG CCACATGCTCTAGTCCACTG 38.1 55.0 55.7 57.7 944-1143 bp 1.0 62.9
psbM-trnR(acg)_p3 GCAGCAAATGAATCTGCTTA CCACATGCTCTAGTCCACTG 40.0 55.0 54.6 57.7 939-1138 bp 1.0 62.7
psbM-trnR(acg)_p4 GCAGCAAATGAATCTGCTTAA CCACATGCTCTAGTCCACTG 38.1 55.0 55.5 57.7 939-1138 bp 1.0 61.3
psbM-trnR(acg)_p5 AAAACAGCAGCAAATGAATCTG CCACATGCTCTAGTCCACTG 36.4 55.0 56.4 57.7 945-1144 bp 1.0 60.8