Hotspot

rpl23-rpl2

SSC · 120969-121728 · 760 bp

Back to Pediastrum

Pi 0.176

MarkerSeek score 83.90

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 121
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	ATTGCACTTTGAGCCAAGTG	TTACGCCCTGCTTTTCCAAT	45.0	45.0	57.2	58.1	1926 bp	0.5	52.9
rpl23-rpl2_p2	ATTGCACTTTGAGCCAAGTG	TGCTGATCTTCCAAGCTGTC	45.0	50.0	57.2	57.9	1768 bp	0.5	52.7
rpl23-rpl2_p3	ATTGCACTTTGAGCCAAGTG	CTGCTGATCTTCCAAGCTGT	45.0	50.0	57.2	57.9	1769 bp	0.5	52.7
rpl23-rpl2_p4	ATTGCACTTTGAGCCAAGTG	ACTTGACCAATTGTTGCCCA	45.0	45.0	57.2	58.2	1878 bp	0.5	51.8
rpl23-rpl2_p5	ATTGCACTTTGAGCCAAGTG	TTTTTCTGGTTTTGCTCCGC	45.0	45.0	57.2	57.8	1387 bp	0.5	51.8