Hotspot

rrf-trnS(gcu)

IRb · 118788-119536 · 749 bp

Back to Pediastrum

Pi 0.218

MarkerSeek score 85.30

Primer available yes

Label rrf-trnS(gcu)

Feature Metadata

label_name: rrf-trnS(gcu)
feature_type: igs
region: IRb
length_bp: 749
variable_sites: 114
pi: 0.218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrf-trnS(gcu)_p1	GTTGGAAGTCTGGCAGGAAA	CGGATTAGCAATCAGTCGCT	50.0	50.0	58.0	58.1	735-832 bp	1.0	90.7
rrf-trnS(gcu)_p2	TGGAAGTCTGGCAGGAAAAC	CGGATTAGCAATCAGTCGCT	50.0	50.0	58.0	58.1	733-830 bp	1.0	90.7
rrf-trnS(gcu)_p3	ACTTGTTGGAAGTCTGGCAG	CGGATTAGCAATCAGTCGCT	50.0	50.0	58.0	58.1	739-836 bp	1.0	90.6
rrf-trnS(gcu)_p4	GACTTGTTGGAAGTCTGGCA	CGGATTAGCAATCAGTCGCT	50.0	50.0	58.0	58.1	740-837 bp	1.0	90.6
rrf-trnS(gcu)_p5	TGGAAGTCTGGCAGGAAAAC	TACGGAGAGAGAGGGATTCG	50.0	55.0	58.0	57.7	781-878 bp	1.0	89.8