Hotspot

trnMf(cau)-chlB

LSC · 90953-91489 · 537 bp

Back to Pediastrum

Pi 0.246
MarkerSeek score 86.20
Primer available yes
Label trnMf(cau)-chlB

Feature Metadata

label_name
trnMf(cau)-chlB
feature_type
igs
region
LSC
length_bp
537
variable_sites
124
pi
0.246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnMf(cau)-chlB_p1 TTTGAACCTGCGACCTTAGG TTCCCATATGAGCAGGACCT 50.0 50.0 57.8 57.8 643-939 bp 1.0 88.2
trnMf(cau)-chlB_p2 TTTGAACCTGCGACCTTAGG GTTCCCATATGAGCAGGACC 50.0 55.0 57.8 57.7 644-940 bp 1.0 87.7
trnMf(cau)-chlB_p3 ATTATGAGCCCCACGAGCTA TTCCCATATGAGCAGGACCT 50.0 50.0 58.3 57.8 623-919 bp 1.0 87.5
trnMf(cau)-chlB_p4 ATTATGAGCCCCACGAGCTA GTTCCCATATGAGCAGGACC 50.0 55.0 58.3 57.7 624-920 bp 1.0 87.0
trnMf(cau)-chlB_p5 TAGGATTATGAGCCCCACGA TTCCCATATGAGCAGGACCT 50.0 50.0 57.6 57.8 627-923 bp 1.0 86.6