Hotspot

atpI-psbJ

LSC · 72519-74565 · 2047 bp

Back to Pediastrum

Pi 0.257

MarkerSeek score 83.70

Primer available yes

Label atpI-psbJ

Feature Metadata

label_name: atpI-psbJ
feature_type: igs
region: LSC
length_bp: 2047
variable_sites: 364
pi: 0.257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-psbJ_p1	TGACCTACAGAAACTTCAGCA	GGAACAGCTGCTATTGGGTT	42.9	50.0	56.9	58.2	2091-2175 bp	1.0	73.8
atpI-psbJ_p2	TGACCTACAGAAACTTCAGCA	TTGGAACAGCTGCTATTGGG	42.9	50.0	56.9	58.2	2093-2177 bp	1.0	73.8
atpI-psbJ_p3	TGACCTACAGAAACTTCAGCAA	GGAACAGCTGCTATTGGGTT	40.9	50.0	57.6	58.2	2091-2175 bp	1.0	71.4
atpI-psbJ_p4	TTGACCTACAGAAACTTCAGCA	GGAACAGCTGCTATTGGGTT	40.9	50.0	57.6	58.2	2092-2176 bp	1.0	71.4
atpI-psbJ_p5	TGACCTACAGAAACTTCAGCAA	TTGGAACAGCTGCTATTGGG	40.9	50.0	57.6	58.2	2093-2177 bp	1.0	71.4