Hotspot

DR496_pgp095-rpoBb

LSC · 55255-57189 · 1935 bp

Back to Pediastrum

Pi 0.244

MarkerSeek score 78.90

Primer available yes

Label DR496_pgp095-rpoBb

Feature Metadata

label_name: DR496_pgp095-rpoBb
feature_type: igs
region: LSC
length_bp: 1935
variable_sites: 386
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp095-rpoBb_p1	TTCGGCTTTTGCTCCTTCTT	AAGTTTGGCTATCGGCGATT	45.0	45.0	58.0	58.0	2682 bp	0.5	59.5
DR496_pgp095-rpoBb_p2	GCGTTTCTTTTGCACTTGGT	AAGTTTGGCTATCGGCGATT	45.0	45.0	58.1	58.0	2743 bp	0.5	59.1
DR496_pgp095-rpoBb_p3	TTCGGCTTTTGCTCCTTCTT	TCGTCGGCTATTTCGGAATC	45.0	50.0	58.0	57.9	2777 bp	0.5	58.8
DR496_pgp095-rpoBb_p4	TTCGGCTTTTGCTCCTTCTT	CTCGTCGGCTATTTCGGAAT	45.0	50.0	58.0	57.9	2778 bp	0.5	58.8
DR496_pgp095-rpoBb_p5	TTCGGCTTTTGCTCCTTCTT	TCAGCTAGCCTAACCGAAGA	45.0	50.0	58.0	57.9	2663 bp	0.5	58.7