Hotspot

DR496_pgp017-ftsH

LSC · 22287-23002 · 716 bp

Back to Pediastrum

Pi 0.384

MarkerSeek score 80.50

Primer available yes

Label DR496_pgp017-ftsH

Feature Metadata

label_name: DR496_pgp017-ftsH
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 270
pi: 0.384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp017-ftsH_p1	CAAAGAGCCGAGCCAAAAAG	AGAGAACCCACGCAAACAAT	50.0	45.0	57.9	58.0	1430 bp	0.5	59.4
DR496_pgp017-ftsH_p2	CTTCGGCAATTTCGGAATCG	AGAGAACCCACGCAAACAAT	50.0	45.0	57.9	58.0	1393 bp	0.5	59.1
DR496_pgp017-ftsH_p3	CTCGGTTATGCCAAAGTGGA	AGAGAACCCACGCAAACAAT	50.0	45.0	57.9	58.0	1465 bp	0.5	59.0
DR496_pgp017-ftsH_p4	TCGGTTATGCCAAAGTGGAG	AGAGAACCCACGCAAACAAT	50.0	45.0	57.9	58.0	1464 bp	0.5	59.0
DR496_pgp017-ftsH_p5	AGCTGCTTCCTATCCCTTCA	AGAGAACCCACGCAAACAAT	50.0	45.0	58.1	58.0	1495 bp	0.5	58.9