Hotspot

DR496_pgp013-DR496_pgp014

LSC · 16798-17394 · 597 bp

Back to Pediastrum

Pi 0.434

MarkerSeek score 73.70

Primer available yes

Label DR496_pgp013-DR496_pgp014

Feature Metadata

label_name: DR496_pgp013-DR496_pgp014
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 141
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp013-DR496_pgp014_p1	TTCTTCTTTCTACGCTCCGC	CTGAACTGATGAAGCGCTCT	50.0	50.0	58.0	58.0	816 bp	0.5	59.9
DR496_pgp013-DR496_pgp014_p2	TTCTTCTTTCTACGCTCCGC	CGGTTCTTCGATTCTTCGGT	50.0	50.0	58.0	58.0	1390 bp	0.5	59.9
DR496_pgp013-DR496_pgp014_p3	TTCTTCTTTCTACGCTCCGC	CGCATCGGTTCAGTACTTCA	50.0	50.0	58.0	58.0	939 bp	0.5	59.9
DR496_pgp013-DR496_pgp014_p4	ACTCGAGTGCGACAATCTTC	CTGAACTGATGAAGCGCTCT	50.0	50.0	58.0	58.0	1445 bp	0.5	59.8
DR496_pgp013-DR496_pgp014_p5	GAACTCGAGTGCGACAATCT	CTGAACTGATGAAGCGCTCT	50.0	50.0	58.0	58.0	1447 bp	0.5	59.8