Hotspot

DR496_pgp069

IRb · 103662-104438 · 777 bp

Back to Pediastrum

Pi 0.373

MarkerSeek score 82.90

Primer available yes

Label DR496_pgp069

Feature Metadata

label_name: DR496_pgp069
feature_type: gene
region: IRb
length_bp: 777
variable_sites: 268
pi: 0.373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
DR496_pgp069_p1	GCAGCAGCAACAATTGGATT	GCGAGGTTAAGGATCGTTGT	45.0	50.0	57.9	58.0	1776-2775 bp	1.0	91.2
DR496_pgp069_p2	TTGTTTTGATGCGCATCGTC	GCGAGGTTAAGGATCGTTGT	45.0	50.0	58.0	58.0	1669 bp	0.5	59.7
DR496_pgp069_p3	CAGTCGGCTATTTCTTCGCT	GCGAGGTTAAGGATCGTTGT	50.0	50.0	58.1	58.0	1242 bp	0.5	59.4
DR496_pgp069_p4	TTGTTTTGATGCGCATCGTC	ATAAATCTTCGCGCTTCGGT	45.0	45.0	58.0	58.1	1598 bp	0.5	59.2
DR496_pgp069_p5	TAACTTCGAAGATAGCGCGG	GCGAGGTTAAGGATCGTTGT	50.0	50.0	57.9	58.0	1476 bp	0.5	59.0