Hotspot

rrn5-rrn4.5

IRa · 129785-129997 · 213 bp

Back to Pecteilis

Pi 0.0516

MarkerSeek score 79.10

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 213
variable_sites: 11
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	330 bp	1.0	82.0
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	328 bp	1.0	82.0
rrn5-rrn4.5_p3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	326 bp	1.0	82.0
rrn5-rrn4.5_p4	AGTATCGTCACCGCAGTAGA	CGAGCCGCTTATCATTACGA	50.0	50.0	58.0	58.0	381 bp	1.0	81.7
rrn5-rrn4.5_p5	TATCGTCACCGCAGTAGAGT	CGAGCCGCTTATCATTACGA	50.0	50.0	58.0	58.0	379 bp	1.0	81.7