Hotspot

ccsA-ndhD

SSC · 114742-114997 · 256 bp

Back to Pecteilis

Pi 0.0275
MarkerSeek score 68.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
256
variable_sites
7
pi
0.0275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGGATCAATCTATTAGGAATCGG TCGGTTGACAAGGTACAAGT 41.7 45.0 57.7 56.7 370-371 bp 1.0 41.7
ccsA-ndhD_p2 GGATCAATCTATTAGGAATCGGGT TCGGTTGACAAGGTACAAGT 41.7 45.0 57.7 56.7 368-369 bp 1.0 41.7
ccsA-ndhD_p3 GGGATCAATCTATTAGGAATCGGG TCGGTTGACAAGGTACAAGT 45.8 45.0 58.3 56.7 369-370 bp 1.0 41.7
ccsA-ndhD_p4 TGGGATCAATCTATTAGGAATCGG TCGGTTGACAAGGTACAAGTT 41.7 42.9 57.7 57.5 370-371 bp 1.0 41.7
ccsA-ndhD_p5 GGATCAATCTATTAGGAATCGGGT TCGGTTGACAAGGTACAAGTT 41.7 42.9 57.7 57.5 368-369 bp 1.0 41.7