Hotspot

ndhF-rpl32

SSC · 112466-112856 · 391 bp

Back to Pecteilis

Pi 0.0281

MarkerSeek score 57.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 391
variable_sites: 11
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGACATAGTAACTGGGAGCG	TCCACCGGACAGTCAAAAAG	55.0	50.0	57.9	58.0	514-526 bp	1.0	80.7
ndhF-rpl32_p2	GGAGCGGAAAAAGGGGTATT	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	500-512 bp	1.0	80.5
ndhF-rpl32_p3	ACATAGTAACTGGGAGCGGA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.8	58.0	512-524 bp	1.0	80.3
ndhF-rpl32_p4	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	919-931 bp	1.0	80.1
ndhF-rpl32_p5	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	923-935 bp	1.0	80.0