Hotspot

rrn5-trnR-ACG

IRb · 107985-108235 · 251 bp

Back to Pecteilis

Pi 0.00797

MarkerSeek score 53.20

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 251
variable_sites: 2
pi: 0.00797
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	370 bp	1.0	77.7
rrn5-trnR-ACG_p2	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	373 bp	1.0	77.7
rrn5-trnR-ACG_p3	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	371 bp	1.0	77.7
rrn5-trnR-ACG_p4	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	374 bp	1.0	77.7
rrn5-trnR-ACG_p5	ATTCTGGTGTCCTAGGCGTA	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.8	57.8	403 bp	1.0	77.4