Hotspot

rpl16-rps3

LSC · 82513-82719 · 207 bp

Back to Pecteilis

Pi 0.0471

MarkerSeek score 71.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 9
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	57.1	58.0	326-342 bp	1.0	78.6
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	TGGATCAGAGAAGGCAGAGT	45.0	50.0	57.1	57.8	332-348 bp	1.0	76.9
rpl16-rps3_p3	ACGAGTCACACACTAAGCAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	56.9	58.0	325-341 bp	1.0	76.8
rpl16-rps3_p4	AACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	45.0	50.0	57.1	58.4	311-327 bp	1.0	76.1
rpl16-rps3_p5	ACGAGTCACACACTAAGCAT	TGGATCAGAGAAGGCAGAGT	45.0	50.0	56.9	57.8	331-347 bp	1.0	75.1