Hotspot

clpP1-psbB

LSC · 70997-71652 · 656 bp

Back to Pecteilis

Pi 0.017

MarkerSeek score 65.60

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 11
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.0	58.2	836-850 bp	1.0	80.4
clpP1-psbB_p2	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	800-814 bp	1.0	80.3
clpP1-psbB_p3	CAAACTGCATCCTCCTCTCC	CCAGCCCAACCAGAAACTAG	55.0	55.0	58.0	58.2	819-833 bp	1.0	80.2
clpP1-psbB_p4	CCAAACTGCATCCTCCTCTC	CCAGCCCAACCAGAAACTAG	55.0	55.0	58.0	58.2	820-834 bp	1.0	80.2
clpP1-psbB_p5	CAAACTGCATCCTCCTCTCC	TGGACAGAAAGCAATCGACC	55.0	50.0	58.0	58.2	783-797 bp	1.0	80.1