Hotspot

rpl33-rps18

LSC · 66560-66743 · 184 bp

Back to Pecteilis

Pi 0.017

MarkerSeek score 66.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 3
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACACCCAATCG	TAATCGATTCGATCCCCCGA	50.0	50.0	56.9	58.1	360-367 bp	1.0	73.0
rpl33-rps18_p2	CGACACAATACACCCAATCG	GGGGTAAACGCCTACGAAAA	50.0	50.0	56.9	58.2	332-339 bp	1.0	72.4
rpl33-rps18_p3	CGACACAATACACCCAATCGA	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	360-367 bp	1.0	70.6
rpl33-rps18_p4	TCGACACAATACACCCAATCG	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	361-368 bp	1.0	70.6
rpl33-rps18_p5	CGACACAATACACCCAATCG	CCGATCCAATTGGGGGTAAA	50.0	50.0	56.9	57.6	344-351 bp	1.0	70.3