Hotspot

petG-trnW-CCA

LSC · 64900-65030 · 131 bp

Back to Pecteilis

Pi 0.0534

MarkerSeek score 75.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 7
pi: 0.0534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ATTTACAATACAGGCGCGGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	244-252 bp	1.0	81.9
petG-trnW-CCA_p2	ATTTACAATACAGGCGCGGT	CAAAACCCGATGTCGTAGGT	45.0	50.0	58.0	57.9	208-216 bp	1.0	81.7
petG-trnW-CCA_p3	CGGTGATCAGTTGGACCTTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	228-236 bp	1.0	80.8
petG-trnW-CCA_p4	CGGTGATCAGTTGGACCTTT	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	192-200 bp	1.0	80.7
petG-trnW-CCA_p5	ATTTACAATACAGGCGCGGT	CGCTCTTAGTTCAGTTCGGT	45.0	50.0	58.0	57.7	242-250 bp	1.0	79.2