Hotspot

trnF-GAA-ndhJ

LSC · 47345-47908 · 564 bp

Back to Pecteilis

Pi 0.0231

MarkerSeek score 71.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 13
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	672-674 bp	1.0	80.3
trnF-GAA-ndhJ_p2	GCACGGAAAATAGTCGGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	723-725 bp	1.0	80.2
trnF-GAA-ndhJ_p3	TACTCTGCTAGGACGATGCA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	740-742 bp	1.0	80.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	662-664 bp	1.0	79.8
trnF-GAA-ndhJ_p5	GCACGGAAAATAGTCGGGAT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	713-715 bp	1.0	79.7