Hotspot

trnS-GCU-trnG-UCC

LSC · 7844-8465 · 622 bp

Back to Pecteilis

Pi 0.0226

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 14
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	691-713 bp	1.0	53.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	690-712 bp	1.0	53.6
trnS-GCU-trnG-UCC_p3	GATCTGGGGAATCGGAGAGA	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	756-778 bp	1.0	53.5
trnS-GCU-trnG-UCC_p4	ATCTGGGGAATCGGAGAGAG	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	755-777 bp	1.0	53.4
trnS-GCU-trnG-UCC_p5	ATCGGAGAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	746-768 bp	1.0	52.7