Hotspot

rps16-trnQ-UUG

LSC · 6010-6415 · 406 bp

Back to Pecteilis

Pi 0.0404
MarkerSeek score 71.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
406
variable_sites
16
pi
0.0404
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 479-480 bp 1.0 82.6
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 490-491 bp 1.0 82.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 483-484 bp 1.0 81.5
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 494-495 bp 1.0 80.9
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CGGTAAGGCAATGGGTTTTG 45.0 50.0 58.1 57.6 512-513 bp 1.0 80.0