Hotspot

trnK-UUU-rps16

LSC · 4179-4850 · 672 bp

Back to Pecteilis

Pi 0.0291
MarkerSeek score 78.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
672
variable_sites
19
pi
0.0291
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 764-765 bp 1.0 69.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 763-764 bp 1.0 69.2
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 763-764 bp 1.0 68.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 762-763 bp 1.0 68.4
trnK-UUU-rps16_p5 TCAGTCGCGGTCTTCTAAAC GTGCTCAACCCACAGGAAC 50.0 57.9 57.7 58.7 845-846 bp 1.0 67.4