Hotspot

ycf3

LSC · 43056-45019 · 1964 bp

Back to Paulownia

Pi 0.00264

MarkerSeek score 45.40

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1964
variable_sites: 11
pi: 0.00264
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TAAAGAAATGGCGCCTTCTT	CCACGAGAGGGAATGAGAAG	40.0	55.0	55.6	57.4	2014-2027 bp	1.0	57.3
ycf3_p2	ATAAAGAAATGGCGCCTTCT	CCACGAGAGGGAATGAGAAG	40.0	55.0	55.4	57.4	2015-2028 bp	1.0	55.4
ycf3_p3	AATAAAGAAATGGCGCCTTCT	CCACGAGAGGGAATGAGAAG	38.1	55.0	56.2	57.4	2016-2029 bp	1.0	53.8
ycf3_p4	ATAAAGAAATGGCGCCTTCTT	CCACGAGAGGGAATGAGAAG	38.1	55.0	56.2	57.4	2015-2028 bp	1.0	53.8
ycf3_p5	AATAAAGAAATGGCGCCTTCTT	CCACGAGAGGGAATGAGAAG	36.4	55.0	56.9	57.4	2016-2029 bp	1.0	51.7