Hotspot

trnN(GUU)-trnR(ACG)

IRa · 130419-130997 · 579 bp

Back to Paulownia

Pi 0.00321

MarkerSeek score 48.00

Primer available yes

Label trnN(GUU)-trnR(ACG)

Feature Metadata

label_name: trnN(GUU)-trnR(ACG)
feature_type: igs
region: IRa
length_bp: 579
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-trnR(ACG)_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	713-720 bp	1.0	79.5
trnN(GUU)-trnR(ACG)_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	750-757 bp	1.0	78.9
trnN(GUU)-trnR(ACG)_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	754-761 bp	1.0	78.7
trnN(GUU)-trnR(ACG)_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	664-671 bp	1.0	78.3
trnN(GUU)-trnR(ACG)_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	791-798 bp	1.0	78.1