Hotspot

ccsA-ndhD

SSC · 115889-116197 · 309 bp

Back to Paulownia

Pi 0.0169

MarkerSeek score 57.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 309
variable_sites: 12
pi: 0.0169
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCAGCAATTGTGGCGTCTA	ACCCTGATTTCGTTCTTTCACT	45.0	40.9	57.8	57.9	441-495 bp	1.0	68.0
ccsA-ndhD_p2	GCAATTGTGGCGTCTATTGG	ACCCTGATTTCGTTCTTTCACT	50.0	40.9	57.8	57.9	437-491 bp	1.0	67.9
ccsA-ndhD_p3	AATTCAGCAATTGTGGCGTC	ACCCTGATTTCGTTCTTTCACT	45.0	40.9	57.6	57.9	443-497 bp	1.0	66.6
ccsA-ndhD_p4	TCAGCAATTGTGGCGTCTAT	ACCCTGATTTCGTTCTTTCACT	45.0	40.9	57.6	57.9	440-494 bp	1.0	66.2
ccsA-ndhD_p5	ATTCAGCAATTGTGGCGTCT	ACCCTGATTTCGTTCTTTCACT	45.0	40.9	58.5	57.9	442-496 bp	1.0	65.7