Hotspot

trnR(ACG)-trnN(GUU)

IRb · 109115-109693 · 579 bp

Back to Paulownia

Pi 0.00321

MarkerSeek score 48.00

Primer available yes

Label trnR(ACG)-trnN(GUU)

Feature Metadata

label_name: trnR(ACG)-trnN(GUU)
feature_type: igs
region: IRb
length_bp: 579
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ACG)-trnN(GUU)_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	713-720 bp	1.0	79.5
trnR(ACG)-trnN(GUU)_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	750-757 bp	1.0	78.9
trnR(ACG)-trnN(GUU)_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	754-761 bp	1.0	78.7
trnR(ACG)-trnN(GUU)_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	664-671 bp	1.0	78.3
trnR(ACG)-trnN(GUU)_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	791-798 bp	1.0	78.1