Hotspot

petA-psbJ

LSC · 64505-65516 · 1012 bp

Back to Paulownia

Pi 0.000847

MarkerSeek score 47.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 2
pi: 0.000847
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1056-1069 bp	1.0	76.0
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1058-1071 bp	1.0	76.0
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1055-1068 bp	1.0	75.1
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1135-1148 bp	1.0	74.0
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1141-1154 bp	1.0	71.3