Hotspot

trnT(UGU)-trnL(UAA)

LSC · 47280-47984 · 705 bp

Back to Paulownia

Pi 0.00379

MarkerSeek score 48.50

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 6
pi: 0.00379
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	754-766 bp	1.0	79.8
trnT(UGU)-trnL(UAA)_p2	GCGATGCTCTAACCTCTGAG	TAATTCCGGGGGTTCTCTGA	55.0	50.0	57.9	57.7	828-840 bp	1.0	79.5
trnT(UGU)-trnL(UAA)_p3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	805-817 bp	1.0	78.8
trnT(UGU)-trnL(UAA)_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	786-798 bp	1.0	78.8
trnT(UGU)-trnL(UAA)_p5	TAGGAAAAAGCCGGCTATCG	TAATTCCGGGGGTTCTCTGA	50.0	50.0	57.8	57.7	879-891 bp	1.0	78.5